Terminator

Part:BBa_K4152053:Design

Designed by: Xiaoyan Yue   Group: iGEM22_TJUSLS_China   (2022-09-28)


AOX1 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In this project, we need to insert this element into the 3'end of the polyclonal site of the expression vector, and this element can be used to test whether the yeast expression system we constructed is correctly inserted into our target gene.


Source

AOX1 terminator originated from the terminator region of the AOX1 gene in Pichia pastoris. The sequence is 5'-GCAAATGGCATTCTGACATCC-3'.

References